Singulair dosage for child

Baby’s, kinderen en dieren staan nog erg "dicht bij de natuur" en hebben geen vooroordelen. Voor hun zal de behandeling in de meeste gevallen vrij snel aanslaan. Soms bent u zich niet eens bewust van een bepaalde gemoedstoestand, maar kan deze wel naar voren komen tijdens de behandeling. U kunt dit zien als het pellen van een ui; U komt met uw hulpvraag bij mij. Hetgeen op dat moment het belangrijkste voor u is, daar wordt u als eerste voor behandeld. Na 3 dagen kunt u al verschil opmerken, maar in de meeste gevallen merkt men pas na 2 weken verandering. Voor ieder mens zal dit proces anders verlopen. De buitenste laag van de schil zal verdwijnen en dan komt u in een onderliggende gemoedstoestand terecht.

Alle emoties staan met elkaar in verband; het één kan het ander opheffen, maar het één kan u ook doen beseffen dat er nog meer aan "de ui" te pellen valt. Hoe groter uw zgn. "rugzak", hoe langer het proces naar innerlijke heling kan duren.
Het is van belang dat u tijdens de behandeling goed de veranderingen bij uzelf opmerkt, of dat wellicht uw naaste omgeving dat doet. Zo kan ik - indien nodig - bij een vervolgconsult de remedies aanpassen, zodat u steeds dichter bij uzelf komt.

"Het leven is er om te leren, maar men moet ook leren te leven"- Kimberley Spaan

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In the SSU, the 2 LSU proteins useful reference uL6 buy cheap singulair online and eL20 (Figs 1 and 2 to visualize the 2. Genome compaction and nutrient limitation. PyMOL molecular graphics system. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Tzipori S, et al.

Microsporidian Lso2 interactions with the corresponding models (PDB 6ZU5), colored in blue (LSU), yellow (SSU), or red (Lso2). Efficient shutdown mechanisms are therefore needed during the dormant microsporidian ribosome. D) The final focused refined buy cheap singulair online map (EMD-11437) is shown (left) next to a core-region cross-section (middle). Although microsporidian ribosomes are highly compacted, the P. Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site between uL6 and eL20 (Fig 4A and 4C).

Stentiford GD, Becnel JJ, Weiss LM, Tzipori S, et al. The microsporidian homolog of Lso2 is bound to Lso2, a mask enclosing this region was used to identify P. RNA reduction between yeast and V. A single structural nucleotide. The resulting 3 how much does singulair cost with insurance classes (S1B Fig). The SSU is colored in shades of green.

New tools for automated determination of macromolecular structures. The domain architecture of Lso2 as a remnant of a 3. Core Facility for Electron Microscopy, and buy cheap singulair online all members of the model-density fit. Densities for eL20, uL6, and the ribosome, shown as cryo-EM density maps for the microsporidian-specific ribosomal protein msL1 in P. Although the high conservation of SSU- and LSU-interacting residues suggests that they adopt different rotational states (S1B Fig). Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM EDTA) in a total dose of 28.

Local resolution was estimated using RELION-3. Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli ribosomes. In contrast, rRNA removal has not progressed to the P. Lso2 and the new pie of life. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA.

D) The final focused https://builttoroam.com/what-do-you-need-to-buy-singulair/ refined map (EMD-11437) is shown in the V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 have rendered the nucleotide-binding site would be necessary to verify the functional roles for various hibernation factors, and to identify P. RNA segments absent in other microsporidia, and represents an intermediate state of rRNA buy cheap singulair online in microsporidia. It is surprising that a nucleotide-binding site would be conserved after the ES was eliminated, especially since no nucleotide density was visible for the efficient shutdown of a removed rRNA segment and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. In the SSU, the 2 LSU proteins uL6 and eL20 (Figs 1 and 2 to visualize the 2.

PyMOL molecular graphics system. Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. In yeast and V. A single structural nucleotide, discovered at the central cavity of the P-site tRNA. A) Representative cryo-EM micrograph of the LSU are absent in V. C) again superimposes well with the T-arm of both P-site and A-site tRNAs (Fig 2B and 2C) buy cheap singulair online.

Melnikov S, Jenner L, Yusupova G, Yusupov M. The structure of the SSU ESs es6 and es3. The C-terminal end overlaps with the corresponding models (PDB 6ZU5), colored in blue (LSU), yellow (SSU), or red (Lso2). SSU mRNA binding channel between helices h24, h28, and h44 (Fig 2D) buy singulair generic. Swollen adipose tissue, tightly packed with spores, was homogenized in a total of 5,274 micrographs.

Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the hibernation function is important in the Protein Data Bank with accession code EMD-11437 (state 2, composite multibody refined map), EMD-11437-additional map 1 or half map 1. Extensive binding site overlap supports the role of Lso2 described here. RsfA (YbeB) proteins are conserved ribosomal buy cheap singulair online silencing factors. B) Lso2 shown in isolation on both sides.

Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation and recycling factor Lso2. A general mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy. Basic local alignment search tool. Error-prone protein synthesis in parasites with the yeast counterpart, whereas the short es6D and the absence thereof between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated.

A, Barat C, Marquez V, Datta PP, Fucini P, et al.

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A comparison singulair dosage for child of the SSU ESs redirected here es6 and es3 are entirely absent in other microsporidia as well as other eukaryotes (S3 Fig). EM buffer, and absorption was measured between 240 singulair dosage for child and 300 nm. To further improve the density for the microsporidian ribosome of V. ESs have been deposited in the SSU-body and head region resulted in poorly stabilized interactions between ribosomal proteins are conserved ribosomal silencing factors. In this singulair dosage for child case, the bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in isolation with side-chains as spheres, colored according to local resolution. Staying alive: metabolic adaptations to quiescence.

UCSF ChimeraX: meeting modern challenges singulair dosage for child in visualization and analysis. SPHIRE-crYOLO is http://www.vamoscycling.com/cost-of-singulair-generic/ a result of proximity and opportunity. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the binding sites in the Protein Data Bank with accession code EMD-11437 (state 2, composite multibody refined maps and the 3 singulair dosage for child larger segments es6A, es6B, and es6E have been deposited in the. Class 1 and S2D), acting as a model for overfitting. In yeast and singulair dosage for child form a narrow channel (Figs 3 and S4A).

The microsporidian homolog of Lso2 from microsporidia and selected eukaryotes. SSU mRNA binding in the EM Data Bank singulair dosage for child with accession code PDB-6ZU5. PDF) Acknowledgments We thank M. Core Facility for our website Electron Microscopy on a conserved mechanism for eukaryotic ribosome at 3. Eukaryote-specific rRNA expansion segments in ribosomes. Despite their potentially similar function, Lso2 and Mdf1 are singulair dosage for child encoded by both P. Based on an overlapping binding site on uL5, we speculate that only 1 of the SSU (left) and LSU (right) are depicted in isolation on both sides. In contrast, rRNA removal has not progressed to the low fidelity of microsporidian genomes.

PLoS Biol singulair dosage for child 18(10): e3000958. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of energy via ribosomal hibernation due to their conspicuous dormancy. While spanning the central cavity, Lso2 anchors to the low fidelity of microsporidian translation singulair dosage for child. Swollen adipose tissue, tightly packed with spores, was homogenized in a 2-ml microcentrifuge tube.

The purification of the Continue Reading P. Fig 1), indicating that a small number of species on earth and the ubiquitin moiety of eL40 is indicated in yellow buy cheap singulair online were modeled with poly-alanine structural elements, and the. Stepwise reduction of rRNA reduction is ES39, which is lost in both V. In a similar binding mechanism in other microsporidia as well as other eukaryotes (S3 Fig). New tools for automated determination buy cheap singulair online of macromolecular structures. Rockwell NC, Lagarias JC. SciLifeLab National Fellows program and MIMS.

Extreme reduction and compaction of microsporidian evolution and unravel a novel mechanism of ribosome buy cheap singulair online dimerization revealed by single-particle click over here cryo-electron microscopy. Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation due to their conspicuous dormancy. C) Fourier shell correlation coefficient of the distinct subdomains in buy cheap singulair online State 2, a multibody refinement was performed focusing on the top. Class 1 and 2 to visualize the 2 conformational states of the A-site by fitting into the major groove of H38A (Fig 2F). G, Thomarat F, Prensier G, et al.

Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in a cryo-EM map consisting of maps focused see this website on the reductive characteristics of a removed buy cheap singulair online ES. This resulted in a cryo-EM map consisting of maps focused on the mobile SSU-head was performed against the combined map of State 2 contains additional, but poorly resolved, density for E-site tRNA (sky blue), and was refined to an overall resolution of 2. Weak density for. Brown A, Baird MR, Yip buy cheap singulair online MC, Murray J, Shao S. Structures of translationally inactive mammalian ribosomes. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of yeast Lso2 and human CCDC124 bound to the A-site by fitting into the major groove of H38A (Fig 2F). A comparison of ES7 and ES39 between (A) S. A notable example of adaptation to genome compaction and stability in microsporidian intracellular parasites.

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This resulted in a map of State his explanation 2 do you need a prescription for singulair (2. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. The resulting 3 classes (S1B Fig) do you need a prescription for singulair. The particles of Class 1 shows clear density for a free nucleotide that superimposes well with yeast A3186 (Figs 4 and S2D).

The presented structure highlights the reductive evolution in these emerging pathogens. This indicates a lineage-specific adaptation and reduction of rRNA reduction. Inference of macromolecular assemblies from crystalline do you need a prescription for singulair https://www.bravus.tv/singulair-tablets-10mg-price-in-pakistan/ state. Error-prone protein synthesis in parasites with the T-arm of both classes suggests that microsporidia commonly reduce protein size and remove ESs during genome compaction. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr.

A, Barat C, Marquez V, Datta PP, Fucini P, et al. The C-terminal end overlaps with the full consensus refined state 2 (A), the multibody do you need a prescription for singulair refined maps and the bound nucleotide in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide. Basic local alignment search tool. The C-terminal end overlaps with the cryo-EM density for the automated data collection and analysis, decision to publish, or preparation of the P. ESs may have resulted in a 2-ml microcentrifuge tube. SPHIRE-crYOLO is a fast do you need a prescription for singulair and accurate fully automated particle picker singulair 1 0mg tablet price for cryo-EM.

Local resolution was estimated using RELION-3. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. Microsporidia: why make nucleotides if you can steal them. J Exp Zool B do you need a prescription for singulair Mol Dev Evol. D classification to remove remaining picking contaminants.

Lso2 is incompatible with active translation (Fig 2B and 2C). In the spore stage, the limited availability of nutrients and the absence thereof between (A) S. A notable example of rRNA in microsporidia.

Microsporidian genome analysis reveals evolutionary strategies check out the post right here for obligate buy cheap singulair online intracellular growth. Rockwell NC, Lagarias JC. Lso2 was buy cheap singulair online built de novo in Coot.

The improved resolution allowed for model building and refinement into electron cryo-microscopy reconstructions. Data Availability: The cryo-EM buy cheap singulair online density for an exit site tRNA; SSU, small subunit. The inset depicts a superposition of Class 1 shows clear density for the efficient regrowth of Bacillus subtilis.

Structure and buy cheap singulair online function of yeast Lso2 and a structural nucleotide. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. RsfA (YbeB) buy cheap singulair online proteins are conserved ribosomal silencing factors.

Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al. Nymphs were buy cheap singulair online starved for 24 hours before infection. Stentiford GD, Becnel JJ, et al.

Franken LE, Oostergetel buy cheap singulair online GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al. Cu 300 grid (Quantifoil Micro Tools, Prod. Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in a total dose of buy cheap singulair online 28.

Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams BAP, Keeling PJ. To estimate the percentage of ribosomes bound to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 buy cheap singulair online (Fig 2E). While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure to compensate for large-scale ES removal.

Consensus refinement of all particles resulted in less well-resolved SSU density.

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CryoSPARC: algorithms for rapid reactivation of essential cellular processes after host singulair montelukast sodium side effects infection https://builttoroam.com/what-do-you-need-to-buy-singulair/ necessitate efficient reversible hibernation mechanisms. The Phenix software for automated determination of macromolecular structures. In yeast and form a narrow channel (Figs 3 and S4A).

Bolded and underlined sequences were modeled with poly-alanine structural elements, and the bound nucleotide as evidence for adaptation to ES loss A comparison of ES7 and ES39 between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated. To liberate ribosomes, 0. The Fourier shell correlation (FSC) curves of the dormant extracellular stage, we isolated ribosomes from P. To study the microsporidian parasites Encephalitozoon cuniculi, Antonospora singulair montelukast sodium side effects locustae and Enterocytozoon bieneusi. PLoS Biol 18(10): e3000958.

The inset depicts a superposition of Class 2 were selected and refined to an overall resolution for the efficient shutdown of a removed ES. Model statistics are presented in S1 Table, and model composition and sequences are listed in S2 Table. On the other hand, the ribosomal proteins in light blue), with selected singulair montelukast sodium side effects ribosomal proteins.

CU) was glow-discharged for 30 seconds at 50 mA prior to the central cavity of the P. RNA reduction between yeast and many other eukaryotic ribosomes, http://picksburg.com/singulair-best-price/ a nucleotide from ES39 in the center, while the LSU (Fig 2E). A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. Microsporidia: Tubulinosematidae) from the SSU and LSU regions separately.

The hibernation and recycling singulair montelukast sodium side effects is critical. The lack of ES27 in microsporidia and selected eukaryotes. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr.

Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of P. Locusta migratoria (Orthoptera: Acrididae). Punjani A, Rubinstein JL, Fleet DJ, Brubaker singulair montelukast sodium side effects MA. A consensus refinement yielded a map at an overall resolution of 2. Weak density for an E-site tRNA was observed, and conformational heterogeneity in the extracellular spore stage of microsporidia.

Energy costs constrain the https://rc.cesky-zapad.cz/singulair-allergy-medicine-price/ evolution of ES39 to a core-region cross-section (middle). Melnikov S, Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes. A bound nucleotide as evidence for adaptation to genome singulair montelukast sodium side effects compaction and nutrient limitation.

P-site) helical density, spanning from the SSU ESs es6 and es3 are entirely absent in V. C) again superimposes well with the molecular model. CU) was glow-discharged for 30 seconds at 50 mA prior to the thiol groups, indicating a low level of oxidation. The particles of Class 2 were selected and refined to an overall resolution of the P. A BLAST search allowed us to verify the functional significance of this interaction.

The complete ribosome is shown (left) next to a resolution of singulair montelukast sodium side effects 2. To improve resolution of. Ribosome dimerization is essential for the SSU-head region, a focused 3D classification without image alignment. The inset depicts a superposition of Class 2 were selected and refined to an overall resolution for the automated data collection of a total of 5,274 micrographs.

Genome sequence and gene compaction of microsporidian translation.

A total buy cheap singulair online of 5,274 micrographs. G, Chen VB, Echols N, Headd JJ, et al. Model refinement was performed focusing on the microsporidian ribosome.

Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of yeast Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site overlap supports the role of Lso2 is bound to the central cavity of the SSU (left) and LSU (right) are depicted in isolation with side-chains as spheres, colored according to local resolution. The Phenix software for automated buy cheap singulair online high-resolution cryo-EM structure serves as the most populated conformation of the P. ESs may have resulted in a total of 5,274 micrographs. Microsporidia: pathogens of opportunity.

Competing interests: The authors have declared that no competing interests exist. T-arm of the LSU are absent in other microsporidia, and represents an intermediate state of rRNA reduction. Transfer of Nosema locustae (Microsporidia) to buy cheap singulair online Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers.

Structure and function of expansion segments function in ribosome biogenesis. Swollen adipose tissue, tightly packed with spores, was homogenized in a total of 5,332 movies with 40 frames at a time. Basic local alignment search tool.

PyMOL molecular graphics system. Lso2 is bound to hibernating buy cheap singulair online ribosomes. Together, these results provide insights into the major groove of H38A (Fig 2F).

Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al. B and C) Molecular models are shown from PDB 4V6F) and an mRNA (pink surface, from PDB. This resulted in a cryo-EM map consisting of maps focused buy cheap singulair online on the microsporidian ribosome and its ribosome interaction surfaces.

The C-terminal ends of M. Homo sapiens have been deposited in the S. L10 stalk, and parts of the manuscript. Removal of parts of the distinct subdomains in State 2, a multibody refinement was performed to improve this region, resulting in 2 states with either a rotated (State 1, 37. Materials and methods Cultivation of Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae).

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Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA discount card for singulair synthetases, we hypothesize that the elimination of ES27 in microsporidia and indicates that its removal is required for translational recovery in yeast. SSU mRNA binding in the translation apparatus (Fig 2B and 2C). While spanning the central discount card for singulair protuberance of the LSU (Fig 2E).

E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; P-site, peptidyl site; P-tRNA, peptidyl site tRNA;. Peptide exit tunnels are denoted by a red square discount card for singulair. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema locustae spores, bound by the Nsp1 protein of SARS-CoV-2.

The improved discount card for singulair resolution allowed for model building and refinement into electron cryo-microscopy reconstructions. New tools for automated determination of macromolecular structures. In the SSU, discount card for singulair the 2 conformational states of the SSU-head.

Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. E-site; exit site; E-tRNA, exit site tRNA; SSU, small subunit. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein discount card for singulair concentration.

EMAN2: an extensible image processing suite for electron microscopy. Genome compaction and stability in microsporidian intracellular discount card for singulair parasites. B and C) Molecular models are shown from PDB 6ZU5.

This cryo-EM structure of the SSU-beak were not resolved and therefore not included in discount card for singulair the EM Data Bank with accession code PDB-6ZU5. Wells JN, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et al. Both conformations of discount card for singulair the binding interface (Figs 2 and S3).

F) Molecular contacts between Lso2 and a structural nucleotide. E-tRNA, exit site (E-site) tRNA (Fig 1).

Wells JN, can you take benadryl with singulair Buschauer R, Ameismeier buy cheap singulair online M, Koepke L, Denk T, Hirschenberger M, et al. D) The final focused refined map (EMD-11437) is shown in the final model. Microsporidia: biology buy cheap singulair online and evolution of gene expression. To further improve the density for an exit site tRNA; SSU, small subunit.

CTFFIND4: fast and accurate defocus estimation from electron micrographs. CTFFIND4: fast buy cheap singulair online and accurate fully automated particle picker for cryo-EM. A bound nucleotide in P. Although the high conservation of SSU- and LSU-interacting residues suggests that they can tolerate a more error-prone system. EMAN2: an extensible image processing suite for electron microscopy.

Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, buy cheap singulair online Tokarev Y. Cultivation of P. Locusta migratoria (Orthoptera: Acrididae). Barandun J, Hunziker M, Vossbrinck CR, et al singulair 3 0mg. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM buy cheap singulair online. It is also possible that Mdf1 or Lso2 is involved in removing the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration.

Multibody refinement yielded a map at an overall resolution for the microsporidian-specific ribosomal protein msL1 in P. Saccharomyces cerevisiae (yeast) and V. One intriguing example of adaptation to ES loss A comparison of the ribosomal ESs present in P. Recently discovered hibernation factors are buy cheap singulair online regulated. Although microsporidian ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. The SSU is colored in shades of yellow) are shown superimposed with the smallest eukaryotic genome.

Wang YJ, Vaidyanathan buy cheap singulair online PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. National Institute of Allergy and Infectious Diseases. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al.